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KMID : 0881720170320020163
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Journal of Food Hygiene and Safety
2017 Volume.32 No. 2 p.163 ~ p.169
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Antimicrobial Activity of Maesil (Prunus mume) Extract against Vibrio vulnificus
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Ha Tae-Man
Jeon Doo-Yung
Lim Hyun-Cheol
Yoon Yeon-Hee
Shin Mi-Yeong
Yoon Ki-Bok
Kim Jung-Beom
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Abstract
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The purpose of this study was to estimate the antimicrobial activity of Maesil (Japanese apricot, Prunus mume) extract against Vibrio vulnificus. The strains tested in the study were 28 V. vulnificus isolates originated from fish, seawater, mud flat and seawater in fish restaurant. The vvhA gene was detected using real-time PCR and biochemical identification expressed above good identification in 28 isolates of V. vulnificus. All of V. vulnificus used in this study was susceptible to tetracycline and chloramphenicol antibiotics. These two antibiotics were considered to be useful for the treatment of patients. Maesil extracts 2.5% and 5% showed antimicrobial activity against V. cholerae NCCP 13589 and V. parahemolyticus NCCP 11143. V. vulnificus isolate and V. vulnificus NCCP 11135 showed growth inhibition at 1.25%, 2.5% and 5% of Maesil extract, respectively. Compared with V. cholerae and V. parahemolyticus, the antibacterial activity of Maesil extract against V. vulnificus was high. The minimum bactericidal concentration of Maesil extract for V. vulnificus was 1.6%. These results revealed that Maesil extract was found to be very useful for inhibiting the growth of V. vulnificus and can be expected to prevent food poisoning caused by V. vulnificus.
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KEYWORD
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Vibrio vulnificus, Maesil (Japanese apricot), Prunus mume, Antimicrobial activity
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